Coding

Part:BBa_K309004:Design

Designed by: Chris Palmer   Group: iGEM10_Queens-Canada   (2010-10-22)

ChR2::eYFP (for use in C. elegans chassis)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 251
    Illegal XhoI site found at 289
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 654


Design Notes

This is an in frame fusion protein.

Source

From pIC518. This part contains both BBa_K309007 (ChR-2) BBa_K309018 (eYFP), however, it is not a digestion ligation assembly of these two parts. They were assembled using PCR stitching to avoid scarring and keep both parts in the same reading frame.

References